By this study anatomical and palynological characters of Cylamen pseud-ibericum which is an endemic spe- cies for Turkey were researched and anatomical features of this species were described for the first time. Since some species of Cyclamen have been used as folk medicines owing to biological activities of their tubers extracts, its tuber extract was analysed by GC-MS (Gas Chromatography-Mass Spectrometry) and qualified according to its chemical composition by this study. The compounds belong to the alcohols, aliphatic acids and their esters, aromatic compounds, carboxylic acids and their esters, hydrocarbons, fatty acids and their ester and sugars groups were determined by chemical analysis of tuber extract. “ 2-hydroxy-6-methyl-3-nitro-pyran- 4-one” was found in highest ratio (17.31%) in the extract. These results can be a step for new uses of Cyclamen pseud-ibericum tubers. Knowingly the chemical content will make easier biological activity tests. The anatomi- cal and chemical results of this study will be guide for pharmacologist and pharmacists to recognise the plant and the chemical composition. So the results will be useful for both taxonomists and pharmacists.