Volume 39 - Issue 2 - 163 - 172

Application of Cu2+-attached Magnetite Nanoparticles Embedded Supermacroporous Monolithic Composite Cryogels for DNA Adsorption

Cu2+-takılı Magnetit Nanopartikül Gömülü Süpermakrogözenekli Monolitik Kompozit Kriyojellerin DNA Adsorpsiyon Uygulaması

Cryogels are known as a novel generation of stationary phases in the separation science, and they have been succesfully used as efficient adsorbents for separation and purification of biomolecules, such as proteins, enzymes, DNA, cell organelles and viruses in downstream processes. In this study, DNA adsorption performance of Cu2+-attached magnetite (Fe3O4) nanoparticles (Cu2+-AMNPs) embedded supermacroporous monolithic composite cryogels were investigated. Firstly, Magnetite nanoparticles (NPs) coated with 3-aminopropyltriethoxysilane (APTES) were prepared by silanization reaction. Then, Cu2+ ions were attached to silanized magnetite NPs through amine groups. After that, Cu2+-AMNPs were embedded into the cryogels for DNA adsorption studies. The prepared cryogel was characterized by scanning electron microscopy (SEM), elemental analysis, FTIR and swelling. SEM analysis indicates that the cryogel have a heteroporous structure with interconnected pores of 10-50 m size, which ascribed to the porogens effect of frozen water crystals. The maximum amount of DNA adsorption was 19.97 mg/g polymer, at pH 8.0. 

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