Metallomics is a rapidly growing research area investigating the interaction of metals with biological mol- ecules (e.g. DNA, proteins and metabolites) in living systems. It aims the understanding of all metal de- pendent metabolic processes such as uptake, transport, storage and excretion at molecular level. In order to access the qualitative and quantitative information of metals, which mostly occur at trace amounts and in the form of non-covalent complexes with biological ligands in a highly complex biological matrix, and to elucidate the metal-dependent life processes, metallomics utilizes the state of the art analytical and spectroscopic tech- niques. The mostly used approach for the analysis of metal complexes by preserving native metal species is the hyphenation of a chromatographic or an electrophoretic technique for high resolution separation with an elemental (e.g., ICP-MS) or molecular (e.g., ESI-MS or MALDI-MS) spectrometry technique for detection and/ or identification. X-ray absorption and X-ray fluorescence spectrometry and in-silico approaches with bioinfor- matics are among other main techniques/methodologies contributing the research activities in metallomics. This study highlights the basic terms, primarily used analytical approaches, state-of-the art instrumental tech- niques and very representative recent applications in the field.