Antibiotics are important agents in the treatment of urinary tract infection (UTI). However, the use of antibiotics is an important risk factor causing antibiotic resistance. Antibiotic inappropriate resistance is one of the most important problems of increased uropathogenic resistance, especially in pediatric urology. Deficiencies in empirical prescription practices can make this problem even worse. In this study, the demographic characteristics of pediatric patients, UTI agents, and antibiotic resistance of these agents were retrospectively evaluated by an automated system. The data from 719 UTI agents isolated from children were separately analyzed for four different age groups (0-2, 2-6, 6-12, and 12-17). The most commonly isolated infection agents were Escherichia coli (68.01%), Klebsiella pneumoniae (19.75%), Klebsiella oxytoca (3.34%),Enterobacter cloacae (2.23%), and Pseudomonas aeruginosa (1.95%). Among the age groups, 0-2 age group was quite diverse in terms of infection agents and antibiotic resistance values of these agents were significantly high in this group (p<0.05). Regarding the antibiotic resistance, the most noteworthy ones were the resistance against ampicillin (70.2%), amoxicillin- clavulanate (49.0%), cefixime (38.2%), and trimethoprim/sulfamethoxazole (37.1%). Our study indicated that children in 0-2 age group were under higher risk in terms of UTI agents and their antibiotic resistance but this risk was reduced with increasing age. Moreover, the ratio of girls with UTI was greater than that of boys within 0-2 age group. There was high resistance against cephalosporin, ampicillin, amoxicillin-clavulanate, and trimethoprim/sulfamethoxazole, whereas the resistance against carbapenem (imipenem, ertapenem and meropenem) was found to be low and there was no significant increase.