Quorum sensing (qs) is the system that microorganisms use to improve common behavior with another population member in their environment. Salt Lake in Turkey that is considered as an extreme environment due to its salt concentration, teems in plankton and bacteria. Especially, it is an important field for halophilic bacteria because it provides suitable growth conditions. In this study, it was determined that 20 halophilic bacteria strain isolated from Salt Lake could produce qs signal molecules which are N-acyl homoserine lactone (HSL) derivatives. For detection of these bacterial signal molecules, thin layer chromatography was utilized by using Chromobacterium violaceum cv026 and Agrobacterium tumefaciens A136 that are biosensor bacteria strains. Genomic analysis of strains that produce signal molecules was carried out via 16s rRNA. Halobacterium salinarium, Salicola sp., Halovibrio, and Halomonas were found to be common in Salt Lake. It was also observed that these strains could produce C6-HSL and C8-HSL signaling molecules and produce biofilms.