In the present study, we have provided a strategy to block staphylococcal protein A (SpA) which acts as a viru- lence factor and increase lysozyme activity against the staphylococcal infection. For this purpose, polymeric antibodious nano IgG has synthesized according to photosensitive micro emulsion technique and developed some binding parts on nano IgG for lysozyme. The bioconjugation method based on aminoacid monomer-prote- in cross-linking applying photosensitation and conjugation approach (ANADOLUCA) on micro and nano-struc- tures by ruthenium-chelate based monomers has used. The antibacterial activities of polymeric nano-IgG and lysozyme cross-linked polymeric nano-IgG have investigated against to Staphylococcus aureus. The effects on S. aureus have determined using dilution plate method and turbidity method. In addition, the effects on S. au- reus of lysozyme cross-linked polymeric nano-IgG have determined using confocal laser scanning microscopy. The biocompatibility of nanobioconjugates has determined by flow cytometry. The results have shown that nanobioconjugates could be used as an effective mimic and antimicrobial agents.