Volume 44 - Issue 3 - 233 - 244

Isolation, Identification and Enzyme Characterization of Lipase Producing Bacteria from Mucus Layer of Oncorhynchus mykiss

Oncorhynchus mykiss’in Yüzey Mukus Tabakasından Lipaz Üretici Bakterilerin İzolasyonu, Tanımlanması ve Enzim Karakterizasyonu


In this study, 13 different bacteria were isolated from the surface mucus layer of Oncorhynchus mykiss (ra- inbow trout). These bacteria were identified as Exiguobacterium sp. (Om1), Acinetobacter sp. (Om2, Om7), Microbacterium sp. (Om3), Arthrobacter sp. (Om4), Sphingobacterium sp. (Om5), Stenotrophomonas sp. (Om6, 10 and 11), Pseudomanas sp. (Om8), Corynebacterium sp. (Om12) Aeromonas sp. (Om14) and Psychrobacter sp. (Om15) based on morphological, physiological, and biochemical characteristics, as well as phylogenetic analy- sis using 16S rDNA sequences. We report that isolate Om15 produces a cold-active lipase enzyme. The lipase enzyme was partially purified from the bacterial supernatant and its specific activity was calculated as 64.393 U g–1. Optimal performance of the enzyme occurred at pH 8.0 and 20°C using p-nitrophenyl dodecanoate as a substrate. SDS-PAGE indicated that the lipase enzyme is composed of 2 subunits 58-60 kDa, and that it is possible that the active lipase enzyme is the heterodimer of the subunits, which was confirmed via Native- PAGE. Furthermore, the lipase activity decreased in response to application of Co2+ and Cu2+ ions; however, no significant difference in the lipase activity was observed via application of other ions. 



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