Volume 43 - Issue 3 - 205 - 211

Comparison of PSA Binding for Prostate Cancer and BPH Using Lectin-Glycoprotein Interactions

Prostat Kanseri için Lektin-Glikoprotein Etkileşimlerini Kullanarak PSA ve BPH Bağlanmalarının Karşılaştırılması

The increased amount of Prostate-specific antigen (PSA) binding capacity is estimated to be associated with the initial concentration of PSA in the serum or cancer-related glycosylation patterns. The aim of this study was to determine tPSA and fPSA binding capacities of mPGMA-HDMA-Con A beads using the serum samples of patients with PCa and BPH having the same concentration of tPSA. Concanavalin A (Con A) is preferred to use as ligand due to the high sugar specificity towards mannose. The initial concentration of the Con A in the medium was changed between 0.25 and 2.5 mg/mL in order to determine the optimum amount of Con A immobilized to the beads. Both tPSA and fPSA adsorption capacity of mPGMA-HDMA-ConA beads performed using the serum sample of patient with BPH were found to be higher than that of PCa. Therefore, the reason of high PSA adsorption capacity observed in patients with BPH could be attribute to the glycosylation changes. 

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