High altitude conditions cause changes in organism functions. Low oxygen concentration, low heat and exposure to ultraviolet rays for a long time trigger these changes. In our study, the effects of exercise at 1055 m and 2500 m on: the levels or activities of malondialdehyde (MDA), creatinine, lactate dehydrogenase (LDH) and aspartate aminotransferase (AST), total protein, glucose, cholesterol were investigated. Twelve healthy individuals were included in the study. They exercised 3 hours a day for 5 days in both altitudes. Blood samples were taken and placed into the tubes with anticoagulants, both at the first day and on the fifth day, at 1055 m and 2500 m altitude before and after exercise, the samples were then centrifuged and separated from their plasma. The resuts indicate that glucose and AST (aspartate aminotransferase) increased at 2500 meters, while MDA (malondialdehyde) decreased. At 1500 meters, glucose and creatinine were found to be released, while AST (aspartate aminotransferase) was decreased. Considering the samples taken 10 days after the volunteers returned from 2500 m to 1055 m, the results were found to approach 2500 m pre-exercise values. When the results of 1055 m and 2500 m were compared to the 1st and 5th days, glucose and creatinine were found to be increased both at 1050 m and at 2500 m. It was observed that LDH (lactate dehydrogenase) increased significantly at 5th of 2500m compared to 1st day. Our results showed that both exercise and high altitude affect muscle serum enzymes and glucose MDA values, but these changes do not pose a risk of disease that would require medical intervention.