Volume 48 - Issue 2 - 147 - 157

Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents

Enginar (Cynara Scolymus) Yapraklarından Klorojenik Asitin Ultrasonik Destekli Ekstraksiyonunun Yüzey Yanıt Metadolojisi ile Optimizasyonu ve Toplam Fenolik İçerikleri

Artichoke leaf (Cynara Scolymus) extracts are widely used in medicine. Chlorogenic acids are the compounds which have antioxidant, antibacterial and antiviral properties. In this study, after the artichoke (Cynara scolymus) leaves were dried, the powdered portions were extracted with water. Extracts were analyzed by HPLC and the results were expressed as ppm. The optimum conditions for extracting chlorogenic acid from Cynara Scolymus were determined by ultrasonic assisted extraction using the Surface Response Method based on Box-Behnken design. The experimental conditions of extraction were determined as extraction time (15-45 minutes), temperature (30-70°C) and ultrasonic power (25-75%). The optimum conditions were determined as 47.65% ultrasonic power, 35.18 minutes, 53.40°C temperature. The maximum yield of chlorogenic acid was determined as 10.05 ppm. Also the total phenolic content was measured by UV -Vis spectrometry in order to see antioxidant activity. Absorbance values of the samples which were taken from each samples were measured at 765 nm in UV spectrometer. Optimum conditions were determined as 47.29% ultrasonic power, 29.67 minutes, 55.33% ethanol concentration. Total phenolic content were determined as 70.24 ppm. According to the obtained results, ultrasonic-assisted extraction (UAE) can be an effective method for the extraction of chlorogenic acid active ingredient.

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