Osteomyelitis is a bone infection caused by microorganisms. Localized efficient drug delivery for treatment osteomyelitis is an important topic. Amoxicillin is one of the antibiotics used for osteomyelitis therapy. Magnetic nanoparticles could achieve localized targeted treatment with the help of magnetic field. Starch is a biodegradable and biocompatible biopolymer and can be used for carrier material for drug delivery. The aim of this study is to develop amoxicillin loaded magnetic nanoparticles for the use of treatment of osteomyelitis. Here, magnetite nanostructures were coated with starch and characterized with FTIR, TGA and SEM. It was seen that nanoparticles were spherical and had a size of 14-36 nm. Amoxicillin loading onto magnetic nanoparticles was performed by adsorption method using various concentrations of drug and 0.483 mg amoxicillin was adsorbed on per mg of nanoparticles. In addition, in vitro drug release at pH 7.4 was obtained as in a controlled manner. As a conclusion, it can be suggested that amoxicillin loaded these nanoparticles could have a potential for drug delivery to osteomyelitis.