Volume 46 - Issue 4 - 495 - 503

Impedimetric Nanobiosensor for the Detection of Sequence-Selective DNA Hybridization

Dizi Seçimli DNA Hibridizasyonu için İmpedimetrik Nanobiyosensör


5-amino-2-mercapto-1,3,4-thidiazole (AMT) enriched gold nanoparticles (AuNPs) modified impedimetric sensors were developed for the electrochemical monitoring of sequence-selective DNA hybridization related to Hepatitis B virus (HBV). AMT-AuNP-PGEs presented more repeatable impedimetric responses and provided a suitable surface for more DNA binding onto AMT-AuNPs-PGE surface. The selectivity of DNA biosensor modified with AMT-AuNP was investigated in the presence of target DNA, or the other DNA sequences; e.g, noncomplementary (NC), or mismatch (MM) DNA sequences. The detection limit was calculated as 0.86 μg/mL.



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