Volume 46 - Issue 4 - 629 - 642

Antioxidative, Antiproliferative and Antiangiogenic Activities of Nigellasativa L. pulp, a Waste Material Remaining from Oil Production

Yağ Çıkarma İşlemi Sonrasında Atık Materyal Olarak Ortaya Çıkan Çörek Otu Posasının Anti-oksidan, Anti-proliferatif ve Anti-anjiyojenik Özellikleri


The aim of this study was to investigate the biological activities such as antioxidative, antimetastatic and antiangiogenic properties of the Nigella sativa L. (NS) pulp, which is a waste material of the oil production process. Following the investigation of the characteristic properties of NS pulp extract with UV-visible (UV-vis), fourier-transform infrared (FTIR) and gas chromatography-mass spectroscopy (GC-MS) measurements, its antioxidative capacity was determined using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Trolox equivalent antioxidant capacity (TEAC) assays. Antiproliferative effects of the pulp extract were tested on both cancerous and non-cancerous cells to examine whether the effects are specific to cell types. Antiangiogenic tests were performed via chick chorioallantoic membrane (CAM) assay. The results showed that the NS pulp extract still maintained its radical scavenging effects and reduced the proliferation of cancer cells more efficiently compared to non-cancerous cells. Furthermore, CAM assays demonstrated that the pulp extract effectively limited vascular endothelial growth factor (VEGF)-stimulated vascularization and development. NS pulp might be used for the suppression of the metastatic cell populations and for the treatment of the diseases particularly like cancer, which progress with pathological angiogenesis. This study highlights that such waste materials from plants can be recycled with various uses in many fields.



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