In this study, the aim was to examine honey samples from different origins with two important methods that are used especially in distinguishing the purity and origin of honey. For this purpose, proline and melissopalynological analyses of honey samples obtained from four different regions of Turkey (Erzurum, Giresun, Hakkari, Rize) were performed. The melissopalynological analyses of the samples were carried out with a light microscope. Proline analysis was performed using the spectrophotometric method. As a result of melissopalynological analyses, samples of monofloral and multifloral flower honey were encountered. The proline ratios of honey samples ranged from 503.46 mg/kg to 696.09 mg/kg. In conclusion, as a result of melissopalynological analyses, proline content of honey samples that were determined to be chestnut honey was generally found to be lower.