Researches investigate new types of scaffolds for tissue engineering and regenerative medicine applications to support cell proliferation and growth as well as tissue repair and regeneration. Although, there are several types of polymeric materials and various kinds of preparation techniques are already used today; there is still no consensus on the answer of the question “what is the best scaffold for tissue repair and regeneration?”. Cryogels, which is a kind of hydrogels and, cryogelation, which is the technique for cryogel preparation, are rather new for tissue engineering applications. Here in this study, dextran and carboxymethyl cellulose functional polysaccharides blended collagen cryogels were prepared and characterized by chemical, structural and biological evaluations. Results show that, these collagen cryogels with their polysaccharides functional components have proper chemical and thermal characteristics and show good bio and hemo- compatibility. Therefore, these cryogels can be used as a candidate scaffold for tissue engineering and regenerative medicine applications.