Acrolein is a very electrophilic α, β aldehyde, formed in foods by thermal processing of amino acids, fats and carbohydrates. Due to lack of toxicological information about acrolein exposure, male rats from weaning to postpubertal period was evaluated in terms of reproductive toxicity. Weaning male rats were orally exposed to 0.5, 1 and 2 mg/kg/day acrolein for 90 days. Serum testosterone and lutenizing hormone (LH) levels were measured and sperm paremeters were evaluated. Reproductive organs were examined histopathologically. Although several histopathological alterations were observed in reproductive organs of rats in acrolein treatment groups, no changes were observed in hormone levels and sperm count.