The main objective of this study is to find an association between hemoglobin (Hb) and Organic acid anhydrides (OAAs). The blood protein Hemoglobin (Hb) is influenced after an exposure to environmental hazardous OAAs compounds in paint & Chemical industry workers occupationally in SITE Area, Kotri, Sindh,Pakistan. A total of 85 workers fulfilled this research study criteria. These workers were divided into two groups like Paint industry (n=40) and chemical industry (n=45) workers. Both worker groups were compared with non-exposed (normal) group for comparative study. The hemoglobin test was done by simple and standard Kit method system on Microlab 300. The data was analysed by using SPSSversion16 software. The mean Hb for paint industry workers (11.5±1.4) and chemical industry workers (12.7±0.7) were lower than non-exposed normal (16.01±0.1) group. Thus, plasma Hb levels were significantly lower among both worker groups. There is prevalence of anemia found due to lower iron deficiency and poor socio-economic conditions among workers after prolonged harmful chemical compounds exposure.