Volume 45 - Issue 2 - 213 - 218

Primary Culture of Lepidopteran Adherent and Suspension Cells from Larval Testes of Bombyx mori

Bombyx mori’nin Larval Testislerinden Elde Edilen Yüzeye Bağımlı ve Süspanse Lepidopter Hücrelerinin Primer Kültürü

In this study, first aim is to establish primary insect cell culture from larval testes of Bombyx mori. The other aim this to investigate the appropriate cells for establishing continuous cell lines. Testes obtained from 5 instar larvae of B. mori were cultured in Grace’s insect medium. Consequently, it was seen that primary cell culture from testes of B. mori resulted in suspension and adherent cell lines. In addition, this study showed that while adherent cells were not appropriate choice to initiate continuous cell line, suspension cells were suitable to establish continuous cell lines from Bombyx mori testes. 

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