The aim of this study was to investigate the biological activities of Satureja boissieri Hausskn. ex Boiss. (Lami- aceae) essential oil. Antimicrobial effects of the major components from the essential oil were determined by agar well and disc diffusion methods. Antiproliferative effect of the essential oil against human colorectal adenocarcinoma (HT-29) cell line was evaluated by Real Time Cell Analyzer xCELLigence method. In addition, total phenolic content and radical scavenging activity of the essential oil were determined. The results show that thymol, thymol 40%:carvacrol 60%, and terpinene-4-ol 25%:carvacrol 75% had significant antimicrobial activity against S. aureus ATCC 25923 and S. aureus ATCC 29213 strains. Also, the essential oil exhibited the high inhibitory effect on the growth of HT-29 cell line (IC50<10 μg/mL).