The focus of present study was to evaluate the potential of Phanerochaete chrysosporium loaded monolithic composite cryogel columns for the removal of Hg2+ ions from aqueous environments. The swelling degree of the composite column was 6.68 g H2O/g whereas the plain cryogel column was observed as 7.12 g H2O/g. Optimum working conditions for the column were determined. The reuse of the column was investigated and the results showed that this specific column can be used heaps of times with observing no decrement the Hg2+ biosorption capacity significantly. Synthetic wastewater studies were also applied and the biosorption capacity for Hg2+ was 75.22 mg/g.