Volume 44 - Issue 1 - 65 - 75

Pectinase Production with Waste Materials by Local Water Isolate of Aspergillus fumigatus Strain 2101 and Purification and Characterization of the Enzyme ​

Yerel Su İzolatı Aspergillus Fumigatus 2101 Suşu Tarafından Atık Malzemeler ile Pektinaz Üretimi ve Enzimin Saflaştırılması ve Karakterizasyonu

In this study, pectin lyase (PL) was obtained from Aspergillus fumigatus strain 2101 which was isolated from local water sources. PL was purified using ammonium sulphate precipitation, gel filtration and ion-exchange chromatography. After these purification steps, 12.4 purification fold was obtained. The optimum pH and tem- perature of PL were 8.0 and 40 ̊C at 70 min, respectively. The molecular weight of PL was 11.5 kDa. Maximum PL activity was obtained with fruit pulps in submerged culture medium. Stability assays showed that the PL was stable till 20 months at 4 ̊C. The highest PL activity was obtained using citrus pectin as carbon source. PL was strongly activated by FeSO4, FeCl3 and ascorbic acid. 

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