This study aims to determine chemical compounds of the propolis samples gathered from different regions of Turkey in geographical region-base. Gas Chromatography-Mass Spectrometry (GC-MS) analysis, was per- formed on 21 propolis samples acquired from different geographical regions of Turkey. Samples were collected from five different geographical regions including Black Sea, Central Anatolia, East Anatolia, Marmarean and Mediterranean Regions (Three different phytogeographic regions: European-Sibaerian, Irano-Turanian and Meditteranean phytogeographic regions). So, by this study the chemical compositions of different propolis samples gathered from three distinct phytogeographical regions will be compared. Accordingly, “1-Nona- decene”, “pinocembrin”, “phenylethyl alcohol”, “2-propen-1-one,1-(2,6-dihydroxy-4-methoxy phenyl)-3-phenyl” and particularly “ 2-methoxy-4-vinyl phenol”, “ethyl oleate” were determined as mostly found compounds in collected propolis samples. According to the GC-MS analysis results, five geographical regions are distin- guished from each other according to chemical compound groups. “Benzoic acid “and “17-Pentatriacontene” were found as possible markers of Black Sea Region, “chrysin” for Marmarean region, “2-buten-1-ol,2-methyl”, “Z-12-pentacosane”, “1-Hexacosane” for Central Anatolia, “Nonadecane”, ”Octadecane”, and “2-Nonadecan- one” for East Anatolia Regions. Besides these for the chemical compositions of Mediterranean Region samples, no clear distinction could be found.