Volume 42 - Issue 3 - 409 - 420

In Vitro Determination of AChE Enzyme Activity from Coenuriasis Ill Sheep to Some Drugs

Coenuriasis Hastalıklı Koyunlarda Asetilkolinesteraz Enzimi Aktiviteleri Üzerine Bazı İlaçların İn Vitro Etkilerinin Araştırılması


In this study, the blood plasma of healthy sheep and those with ‘‘coenuriasis’’, (popularly known as ‘‘gid’’), were examined and a comparison of the activities of brain homojenizat acetylcholinesterase have been made. Lastly, a kinetic study was done. The effects of drugs such as Ricobendazole, Amikacin, Gentamicine, Clindamicine and Ceftriaxone, on both healthy and diseased sheep’s enzymes were examined. The drugs Ricobendazole and Clindamicin can inhibit the symptoms but it was observed that the other drugs had no effect. While the I50 blood plasma value of Ricobendazole and Clindamicin drugs over the healthy sheep were 10.19x10-3 M and 36.14x10-3 M, the values of these drugs over the unhealthy sheep were found as 18.51x10-3 M and 6.90x10-2 M. Further K value of Ricobendazole and Clindamicine drugs over the healthy sheep were 3.00x10-8 M and 7.36x10-7 M respectively and the same values of these drugs over the unhealthy sheep were found as 2.62x10-8 M and 8.50x10-7 M. The I50 value of Rizkobendazol and Clindamicine drugs over the healthy sheep’s brain were 6.27x10-7 M and 7.81x10-7 M, and the same values of these drugs over the unhealthy sheep’s brain were found as 27.75x10-7 and 7.67x10-7. Finally, K value of Rikobendazol and Clindamicine drugs over the healthy sheep’s brains were 3.06x10-8 M and 1.82x10-6 M and the same values of these drugs over the unhealthy sheep’s brains were found as 1.02x10-7 M and 1.95x10-6 M. Changes in the enzyme activity of brain groups was statistically significant (p<0.05). 



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