Volume 42 - Issue 3 - 387 - 393

Cloud Point Extraction and Determination of Cyclopiazonic Acid and Tenuazonic Acid in Tomato Juice

Domates Suyundaki Siklopiazonik Asit ve Tenuazonik Asitin Bulutlanma Noktası Ekstraksiyonu ve Tayini


Acloud point extraction as a preconcentration technique was developed for the determination of a tenua- zonic and a cyclopiazonic acid in tomato juices. These mycotoxins were extracted and preconcentrated using Triton-X 114 as surfactant and determined by high performance liquid chromatography with ultraviolet detection. The optimized extraction conditions were pH 2, 4% Triton X-114 (w/v), 1% KNO3 (w/v), 50°C as the extraction temperature and 30 min for extraction time. The extraction recoveries were found as 40% and 95% for tenuazonic acid and cyclopiazonic acid, respectively. The linearity of the proposed method was in the concentration range of 10 to 2000 ng mL-1 for both mycotoxins. 



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