Volume 42 - Issue 2 - 225 - 231

In vitro evaluation of various crude extracts of seeds of Trachyspermum Ammi (L.) Sprague (Ajwain) for their potential inhibitory action on selected bacteria of clinical significance

Trachyspermum Ammi (L.) Sprague (Ajwaın) tohumlarından elde edilen çeşitli ham özütlerin klinik olarak önemli olan bazı bakterilere karşı potansiyel inhibisyon etkilerinin in vitro olarak test edilmesi

Seeds of Trachyspermum ammi (L.) Sprague (Ajwain) were evaluated for their antibacterial potential against several bacteria of clinical significance viz. Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus, Bacillus subtilis, Escherichia coli and Salmonella typhi. Extracts were found to inhibit one or more bacterial strains in different concentrations as observed by microbroth double dilution methodology. The pattern of inhibition depends largely on the solvent used for extraction and the organism tested. Extracts prepared in organic solvents were found more active than aqueous extracts. Furthermore, methanol extract was found to have greater activity against almost all the bacterial species tested. Gram positive bacteria were found more sensitive when compared to Gram negative bacteria. Staphylococcus aureus was found to be most susceptible followed by Bacillus species. Salmonella typhi and Streptococcus pyogenes were found to be most resistant bacteria, however, Escherichia coli showed mild sensitivity to some of the extracts. The study reveals the possibility of the presence of antibacterial components in the Ajwain seeds, thus it can assure an interesting future prospect in the world of medicine for the discovery of novel agents with antimicrobial potential. 

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