In this study, we assessed the antimicrobial resistance patterns of methicillin-resistant Staphylococcus aureus (MRSA) isolates obtained from three different hospitals in Ankara, detected the occurrence of multidrug- resistance among MRSA using agar disc diffusion test. The greatest resistance was observed against ampicillin, amoxicillin-clavulanic acid, penicillin and tetracycline (fully resistant), followed by ciprofloxacin (98%), gentamicin and rifampicin (96%), clindamycin and eritromycin (72%). The least resistance was observed against trimethoprim-sulfamethoxazole (4%), fusidic acid (2%), whereas vancomycin showed no resistance. For MRSA isolates, multidrug-resistance was common and only few antibiotics were active against these isolates. Therefore, successive surveillance on antibiotic susceptibility of MRSA is necessary for the determination of emerging trends and the development of appropriate therapeutic strategies.