Developing of new techniques for detecting important biologic molecules are one of the aims of molecular imprinting. Serotonin (5-hydroxytryptamine) is a biogenic amine that acts as a neurotransmitter in the central and peripheral nervous systems. It is present in a variety of organisms, ranging from humans to species such as worms that have primitive nervous systems, and mediates a variety of physiological responses in distinct cell types. Serotonin is one of the indolic compound which has a clinical importance. Serotonin imprinted polymers were synthesized by using different functional monomers. Best results were taken with 2-(trifluoromethyl) acrylic acid and methacrylamide. The binding capacities in different solvents were evaluated. The specifity of polymer was tested by real blood sample.